Open source DIY kit¶
UV-transilluminators are used in molecular biology labs to view DNA (or RNA) that has been separated by electrophoresis through an agarose gel. During or immediately after electrophoresis, the agarose gel is stained with a fluorescent dye which binds to nucleic acid. Exposing the stained gel to a UVB light source causes the DNA/dye to fluoresce and become visible. This technique is used wherever the researcher needs to be able to view their sample, for example sizing a PCR product, purifying DNA segment after a restriction enzyme digest, quantifying DNA or verifying RNA integrity after extraction.
The open source UV Transilluminator kit can be used to make your own narrowband UV-B transilluminator with a 7.5 x 7.5 cm viewing window suitable for imaging DNA mini-gels. The kit contains all of the materials for making the transilluminator. Assembly time is approx. 30-60 mins. No additional tools are required.
- Kit: Cat # IMG-01, $225 at www.iorodeo.com.
- Enclosure: 19.8 cm long x 14.9 cm wide x 6.5 cm tall
- Viewing window: 7.5 cm x 7.5 cm
- Output wavelength: 305-315 nm with a peak at 311 nm. See attached spec sheet for more information on the UVB bulb
- Kit Contents
- Acrylic enclosure parts
- Enclosure Hardware
- Assembly Step 1: Connect Power
- Assembly Step 2: Enclosure base assembly
- Assembly Step 3: Enclosure top assembly
- Assembly Step 4: Attach the lid
- Viewing a gel with the UV Transilluminator
- Open source design files